Quantification of Interaction between Human Skin Fibroblasts and Collagen by Image Texture Analysis
Abstract: Collagen plays an important role in maintaining healthy structures in human skin.
Fibroblasts are the cells located in collagen-rich skin dermis and they are responsible for producing
collagen of connective tissue. However, the effects of coating of the culture dish with collagen on
fibroblast culture are still unknown. Therefore, to uncover the effects, we propose a quantitative
method based on Gray-Level Co-occurrence Matrix. This method computes two texture similarity
indices, called “Texture Mean Distance (TMD)” and “Texture Mean Spread (TMS)” by integrating
texture features from images. An experiment with two sets of microscope cell culture images with and without collagen coating demonstrates promising performances of the metrics to identify the effects of collagen on human fibroblasts.
Keywords: cellular images, human skin fibroblasts and collagen, statistical texture features, texture mean distance, texture mean spread