Abstract: Neural interface is man-made information pathway through which biological nerve system could communicate directly with electromechanical devices including computer, robot and even cyborg. This paper introduces a novel neural interfacing electrode array capable of bidirectional information transmission and multi-signals recording. The novel flexible electrode array with 32 channels enables close-looped control and feedback neural interfacing researches with the capability of both electrical stimulation and simultaneous recording of electroencephalogram (EEG), electromyogram (EMG), electroneurogram (ENG) signals. The electrochemical impedance spectroscopy (EIS) measurement of the electrode array was carried out to evaluate the electrode array electrochemical performance in the electrophysiology frequency range. Electrical stimulation to peripheral nerve was performed with various stimulation configuration of ENG electrode site pairs to produce distinct activation patterns. Muscle action potentials of the gastrocnemius of the hind limb indicated different configuration of electrode site pairs could generate distinct stimulating effect. In addition, three groups of in vivo experiments were conducted to demonstrate the recording ability of the electrode array for nerve signals. The data from 32 channels verified the effectiveness of this flexible electrode array in simultaneous recording of EEG/EMG/ENG in vivo.